Cryopreservation yog khov ntawm cov txiv neej thiab poj niam cov kab mob, nrog rau embryos rau lub hom phiaj ntawm khaws cia rau lawv rau lub sijhawm tsis tu ncua. Phev, oocytes thiab embryos yog zoo rau kev khov tob (txog -196 degrees Celsius) hauv cov kua nitrogen.
Ua ntej khov, tag nrho cov dej noo tawm ntawm lub hlwb, vim nws yog kev tuag thaum khov. Khaws cov khoom khov rau hauv cov reservoirs tshwj xeeb - Dewar vessels. Tag nrho cov feem sau npe, embryos yog cia 1-2 vitro.
Dab tsi yog cryopreservation phev thiab oocytes?
Phev tuaj yeem tso tau yog tias IVF npaj, tab sis hnub ntawm tus txiv neej tsis tuaj yeem nyob hauv qhov chaw kho mob rau ib qho laj thawj lossis lwm qhov. Ib qho kev phem phev yog ib qhov laj thawj ntxiv rau kev mob rau cryopreservation ntawm spermatozoa. Qhov no ua rau nws ua tau nyob hauv ntau theem kom sau tau cov phev zoo thiab muaj peev xwm ua tiav ib qho kev pab cuam ntawm nyob hauv vitro fertilization.
Lub qe yuav khov rau cov neeg uas muaj kab mob oncological. Ua ntej lub tshuab hluav taws xob thiab chemotherapy, uas feem ntau ua rau kev poob ntawm fertility rau cov poj niam , koj tuaj yeem ua rau lub Tshuag ntawm cov qe kom yav tom ntej nws muaj peev xwm muaj me nyuam yaus.
Khov phev
Sau rau khov phev daim tshev. Nws muaj kev ywj pheej thiab kev ua kom tiav. Yog tias cov ntsuas tau zoo, thiab kev sim seb puas muaj peev xwm khov cov phev tau ua tiav, cov ntaub ntawv tshiab sau ua kom tuab thiab ua kom cov concentration, ua kom zoo thiab muab tso rau hauv lub khob. Lub khob ntim quav yog lub taub yas me me. Cov raj no tau sau lo lus kom tsis txhob yuam kev thaum uas fertilized nrog cov phev khov.
Cryopreservation ntawm oocytes
Sau los ntawm cov qe rau khov yog ntev thiab nyuab ntau dua cov phev. Ib tug poj niam yog ib qho kev ua kom hormonal ntawm lub zes qe menyuam kom ntau oocytes paub tab tib lub sijhawm. Tom qab no, muaj ib qho kev qe ntawm qe, xaiv ntawm feem ntau siv tau. Lawv kho thiab cov kua ntau dhau lawm, muab tso rau hauv ib lub thawv tshwj xeeb thiab khov nrog kua nitrogen.
Cryopreservation ntawm embryos
Embryos yog khov rau ntau lub hom phiaj. Xub thawj, thaum ua li ntawd, thaum muaj ib qho kev sim ua tsis tiav, IVF yuav raug rov sim dua yam tsis rov ua kom rov qog cov zes qe menyuam thiab ua rau cov oocytes.
Tsis tas li ntawd, embryos undergo cryopreservation nyob rau hauv rooj plaub uas tus poj niam tsis kam hloov lub npe ntawm embryos (ovarian hyperstimulation syndrome). Immaturity ntawm endometrium yog lwm cov laj thawj rau khov embryos. Embryos nyob rau hauv cov ntaub ntawv no tsim ob peb hnub, ces qhov zoo tshaj plaws ntawm lawv yog xaiv thiab txaus ntseeg kom khov. Thaum tus poj niam endometrium npaj txhij rau embryos, qhov hloov ntawm frozen embryos yuav siv qhov chaw.
Frozen embryo yog cia li ntev raws li koj xav tau. Ntawm chav kawm, txheej txheem ntawm khov thiab defrosting yog kev nyuab siab rau embryos. Tab sis niaj hnub txoj kev tso cai tsis tsuas yog kom txuag tau ntau dua cov khov embryos hauv lub xeev uas siv tau, tab sis kuj yuav lav lawv cov qub
Li mob rau cryopreservation ntawm embryos
Ua ntej, tsuas yog qhov zoo tshaj plaws embryos muab rau hauv Frost - nrog lub siab tshaj plaws zoo indicators. Qhov thib ob, nws tshwm sim ntawm ib theem ntawm lawv txoj kev loj hlob: hauv theem 2, 4, 8 hlwb thiab blastocysts.
Embryos, nws cov cim qhia tias nws tsis zoo, tsis txhob qiv tus kheej kom khov, raws li lawv muaj cov cuab yeej ntawm kev saib tsis taus - rhuav tshem. Qee lub sij hawm zoo embryos yog tseem rhuav tshem - qhov no yog qhov nqi rau lawv khov thiab tom qab defrosting. Tab sis muaj ib txwm ob peb embryos khov, yog li ib txhia ntawm lawv nyob tom qab defrosting.